Usage: fastq-dump [options] [ -A ] <accession> fastq-dump [options] <path> INPUT -A|--accession <accession> Replaces accession derived from <path> in filename(s) and deflines --table <table-name> (New) Table name within SRA format, default is SEQUENCE PROCESSING Read Splitting Sequence data may be used as raw or split into individual reads --split-spot Split spots into individual reads Full Spot Filters Applied to the full spot independently of --split-spot -N|--minSpotId <rowid> Minimum spot id -X|--maxSpotId <rowid> Maximum spot id --spot-groups <[list]> Filter by SPOT_GROUP (member): name[,...] -W|--clip Apply left and right clips Common Filters Applied to spots when --split-spot is not set, otherwise - to individual reads -M|--minReadLen <len> Filter by sequence length >= <len> -R|--read-filter <[filter]> Split into files by READ_FILTER value optionally filter by a value: pass|reject|criteria|redacted -E|--qual-filter Filter used in early 1K Genomes data: no sequences starting or ending with >= 10N Filters for individual reads Applied only with --split-spot set --skip-technical Dump only biological reads OUTPUT -O|--outdir <path> Output directory, default is '.' Multiple File Options Setting this options will produce more than 1 file, which will be suffixed by splitting criteria. --split-files Dump each read into a separate file.Files will received suffix corresponding to read number --split-3 Legacy 3-file splitting for mate-pairs: First 2 biological reads satisfying dumping conditions are placed in files *_1.fastq and *_2.fastq If only 1 biological read is dumpable - it is placed in *.fastq Biological reads 3 and above are ignored. -G|--spot-group Split into files by SPOT_GROUP (member name) -R|--read-filter <[filter]> Split into files by READ_FILTER value optionally filter by a value: pass|reject|criteria|redacted -T|--group-in-dirs Split into subdirectories instead of files -K|--keep-empty-files Do not delete empty files FORMATTING Sequence -C|--dumpcs <[cskey]> Formats sequence in color space (default for SOLiD),cskey may be specified for translation -B|--dumpbase Formats sequence in base sequence (default for other than SOLiD). Quality -Q|--offset <integer> Offset to use for quality conversion, default is 33 --fasta Fasta only, no qualities Defline -F|--origfmt Defline contains only original sequence name -I|--readids Append read id after spot id as 'accession.spot.readid' on defline --helicos Helicos style defline --defline-seq <fmt> Defline format specification for sequence. --defline-qual <fmt> Defline format specification for quailty. <fmt> is string of characters and/or variables. Variables could be are one of: $ac - accession, $si - spot id, $sn - spot name, $sg - spot group (barcode), $sl - spot length in bases, $ri - read number, $rn - read name, $rl - read length in bases. '[]' could be used for an optional output: if all vars in [] yield empty values whole group is not printed. Empty value is empty string or 0 for numeric variables. Ex: @$sn[_$rn]/$ri - '_$rn' is omitted if name is empty OTHER: -h|--help Output a brief explantion for the program -V|--version Display the version of the program then quit -L|--log-level <level> Logging level as number or enum string One of (fatal|sys|int|err|warn|info) or (0-5) Current/default is warn -v|--verbose Increase the verbosity level of the program Use multiple times for more verbosity