5.1.1. annotate

bedtools annotate, well, annotates one BED/VCF/GFF file with the coverage and number of overlaps observed from multiple other BED/VCF/GFF files. In this way, it allows one to ask to what degree one feature coincides with multiple other feature types with a single command.

5.1.1.1. Usage and option summary

Usage:

bedtools annotate [OPTIONS] -i <BED/GFF/VCF> -files FILE1 FILE2 FILE3 ... FILEn

(or):

annotateBed [OPTIONS] -i <BED/GFF/VCF> -files FILE1 FILE2 FILE3 ... FILEn

Option	Description
-names	A list of names (one per file) to describe each file in -i. These names will be printed as a header line.
-counts	Report the count of features in each file that overlap -i. Default behavior is to report the fraction of -i covered by each file.
-both	Report the count of features followed by the % coverage for each annotation file. Default is to report solely the fraction of -i covered by each file.
-s	Force strandedness. That is, only include hits in A that overlap B on the same strand. By default, hits are included without respect to strand.
-S	Require different strandedness. That is, only report hits in B that overlap A on the _opposite_ strand. By default, overlaps are reported without respect to strand.

5.1.1.2. Default behavior - annotate one file with coverage from others.

By default, the fraction of each feature covered by each annotation file is reported after the complete feature in the file to be annotated.

$ cat variants.bed
chr1 100  200   nasty 1  -
chr2 500  1000  ugly  2  +
chr3 1000 5000  big   3  -

$ cat genes.bed
chr1 150  200   geneA 1  +
chr1 175  250   geneB 2  +
chr3 0    10000 geneC 3  -

$ cat conserve.bed
chr1 0    10000 cons1 1  +
chr2 700  10000 cons2 2  -
chr3 4000 10000 cons3 3  +

$ cat known_var.bed
chr1 0    120   known1   -
chr1 150  160   known2   -
chr2 0    10000 known3   +

$ bedtools annotate -i variants.bed -files genes.bed conserve.bed known_var.bed
chr1  100     200     nasty   1       -       0.500000        1.000000        0.300000
chr2  500     1000    ugly    2       +       0.000000        0.600000        1.000000
chr3  1000    5000    big     3       -       1.000000        0.250000        0.000000

5.1.1.3. -count Report the count of hits from the annotation files

$ bedtools annotate -counts -i variants.bed -files genes.bed conserve.bed known_var.bed
chr1  100     200     nasty   1       -       2       1       2
chr2  500     1000    ugly    2       +       0       1       1
chr3  1000    5000    big     3       -       1       1       0

5.1.1.4. -both Report both the count of hits and the fraction covered from the annotation files

$ bedtools annotate -both -i variants.bed -files genes.bed conserve.bed known_var.bed
#chr  start   end     name    score   +/-     cnt1    pct1    cnt2    pct2    cnt3    pct3
chr1  100     200     nasty   1       -       2       0.500000        1       1.000000        2       0.300000
chr2  500     1000    ugly    2       +       0       0.000000        1       0.600000        1       1.000000
chr3  1000    5000    big     3       -       1       1.000000        1       0.250000        0       0.000000

5.1.1.5. -s Restrict the reporting to overlaps on the same strand.

$ bedtools annotate -s -i variants.bed -files genes.bed conserve.bed known_var.bed
chr1  100     200     nasty   1       -       0.000000        0.000000        0.000000
chr2  500     1000    ugly    2       +       0.000000        0.000000        0.000000
chr3  1000    5000    big     3       -       1.000000        0.000000        0.000000

5.1.1.6. -S Restrict the reporting to overlaps on the opposite strand.

$ bedtools annotate -S -i variants.bed -files genes.bed conserve.bed known_var.bed
chr1  100     200     nasty   1       -       0.500000        1.000000        0.300000
chr2  500     1000    ugly    2       +       0.000000        0.600000        1.000000
chr3  1000    5000    big     3       -       0.000000        0.250000        0.000000

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